Can I be alone with a coworker of the opposite sex?

No, you should avoid being truly alone with a coworker of the opposite sex. Islam forbids khalwa: a man and a woman who aren't mahram being alone in a private, closed setting. Working together in shared or visible spaces is fine, so small changes usually solve the problem.
What khalwa means
The Prophet ﷺ said, "No man should be alone with a woman except in the presence of a mahram" (Sahih al-Bukhari 5233). Khalwa means being alone together where nobody else can see or walk in, like a locked office, a closed meeting room, or a home. The rule isn't about suspecting you or your colleague. It's a protective fence, in line with the Quran's command to stay far from anything that leads to zina:
A mahram is a close relative you can never marry. See What is a mahram?
What is not khalwa
- Working side by side in an open-plan office.
- A meeting room with a glass wall or an open door, where people pass by.
- A café, canteen or other public place.
- Video calls and phone calls about work (keep them professional).
- A meeting with three or more people.
Common work situations and fixes
- One-to-one with your manager: ask for a glass-walled room, keep the door open, or invite a third colleague.
- Lifts: a short ride in a public lift is not khalwa according to many scholars, but you can wait for the next one if you feel uneasy.
- Car rides: driving alone together is khalwa. Suggest a taxi, public transport or a third passenger.
- Late shifts or closing up: ask to be paired with a same-sex colleague or a third person.
- Business trips: separate rooms, and avoid working in each other's hotel room.
Students face the same question. See Can I study alone with a classmate of the opposite gender?
How to explain it at work
You don't need a long speech. Try: "For religious reasons I prefer not to have closed-door meetings one-on-one with the opposite sex. Could we keep the door open?" Many people find this reassuring, and some workplaces already have open-door policies for safeguarding. If you slip up or it happens unavoidably, don't panic: keep it short and professional, and adjust next time.
- Being alone in a private, closed setting with a non-mahram is not allowed.
- Open-plan offices, glass rooms and public places are not khalwa.
- Use open doors, a third person or video calls to avoid it.
- A short, polite explanation is usually respected.
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